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Addgene

pcDNA-miR-155 toehold switch
(Plasmid #184755)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184755 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA 3.0
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Green Fluorescence Protein
  • Tag / Fusion Protein
    • miR-155 toehold (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CMV
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-miR-155 toehold switch was a gift from Allen Liu (Addgene plasmid # 184755 ; http://n2t.net/addgene:184755 ; RRID:Addgene_184755)

  • For your References section:

    A Novel Synthetic Toehold Switch for MicroRNA Detection in Mammalian Cells. Wang S, Emery NJ, Liu AP. ACS Synth Biol. 2019 May 17;8(5):1079-1088. doi: 10.1021/acssynbio.8b00530. Epub 2019 May 6. 10.1021/acssynbio.8b00530 PubMed 31039307
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