TTR C10S S85C G83R
(Plasmid #184747)

• Purpose: bacterial expression of human TTR mutants
• Depositing Lab: Peter Wright
• Publication: Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764. doi: 10.1021/acs.biochem.1c00073. Epub 2021 Mar 1.

Backbone

• Vector backbone: pet29
• Backbone manufacturer: emdmillipore
• Backbone size w/o insert (bp): 5371
• Total vector size (bp): 5758
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TTR
• Species: H. sapiens (human)
• Insert Size (bp): 387
• Mutation: C10S, S85C, G83R
• Entrez Gene: TTR (a.k.a. ATTR, CTS, CTS1, HEL111, HsT2651, PALB, TBPA, TTN)
• Promoter: T7

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We made mutations on the wild type construct received from the Kelly lab. Description of wild type TTR plasmid generation in supplementary info of this reference : https://pubs.acs.org/doi/abs/10.1021/cb5004477

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry