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pFS_1142_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader1-ARRAY1-DR1-FaqI_Term_NotI
(Plasmid #184742)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184742 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET30b+
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3700
  • Total vector size (bp) 6600
  • Modifications to backbone
    removal of BbsI restriction sites
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    FsRT-Cas1(opt)-Cas2(opt)
  • Species
    Fusicatenibacter Saccharivorans
  • Insert Size (bp)
    2200
  • Mutation
    sequence codon optimized for expression in E. coli
  • Promoter pTetA

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)
  • 5′ sequencing primer CCTTTCTGCGTTTATATACTAGAGTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFS_1142_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader1-ARRAY1-DR1-FaqI_Term_NotI was a gift from Randall Platt (Addgene plasmid # 184742 ; http://n2t.net/addgene:184742 ; RRID:Addgene_184742)

  • For your References section:

    Noninvasive assessment of gut function using transcriptional recording sentinel cells. Schmidt F, Zimmermann J, Tanna T, Farouni R, Conway T, Macpherson AJ, Platt RJ. Science. 2022 May 13;376(6594):eabm6038. doi: 10.1126/science.abm6038. Epub 2022 May 13. 10.1126/science.abm6038 PubMed 35549411
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