pPBpuro-4493NES(Booster-PKA)
(Plasmid
#184709)
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PurposePKA biosensor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184709 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPBpuro
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 9600
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBooster-PKA
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SpeciesSynthetic
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Insert Size (bp)3600
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPBpuro-4493NES(Booster-PKA) was a gift from Michiyuki Matsuda (Addgene plasmid # 184709 ; http://n2t.net/addgene:184709 ; RRID:Addgene_184709) -
For your References section:
Calcium transients trigger switch-like discharge of prostaglandin E(2) in an extracellular signal-regulated kinase-dependent manner. Watabe T, Yamahira S, Takakura K, Thumkeo D, Narumiya S, Matsuda M, Terai K. eLife. 2024 Jan 26;12:RP86727. doi: 10.7554/eLife.86727. 10.7554/eLife.86727 PubMed 38276879