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Addgene

hDHFR
(Plasmid #184707)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184707 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    emdmillipore
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 6000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    DHFR
  • Alt name
    dihydrofolate reductase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    566
  • GenBank ID
    NP_000782.1
  • Entrez Gene
    DHFR (a.k.a. DHFR1, DHFRP1, DYR)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hDHFR was a gift from Peter Wright (Addgene plasmid # 184707 ; http://n2t.net/addgene:184707 ; RRID:Addgene_184707)

  • For your References section:

    Identification of endogenous ligands bound to bacterially expressed human and E. coli dihydrofolate reductase by 2D NMR. Bhabha G, Tuttle L, Martinez-Yamout MA, Wright PE. FEBS Lett. 2011 Nov 16;585(22):3528-32. Epub 2011 Oct 20. 10.1016/j.febslet.2011.10.014 PubMed 22024482
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