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Addgene

pEGFR-miRFP670nano3
(Plasmid #184673)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184673 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pN1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFR-miRFP670nano3
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    4101
  • Promoter CMV
  • Tag / Fusion Protein
    • miRFP670nano3 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFR-miRFP670nano3 was a gift from Vladislav Verkhusha (Addgene plasmid # 184673 ; http://n2t.net/addgene:184673 ; RRID:Addgene_184673)

  • For your References section:

    Single-domain near-infrared protein provides a scaffold for antigen-dependent fluorescent nanobodies. Oliinyk OS, Baloban M, Clark CL, Carey E, Pletnev S, Nimmerjahn A, Verkhusha VV. Nat Methods. 2022 May 23. pii: 10.1038/s41592-022-01467-6. doi: 10.1038/s41592-022-01467-6. 10.1038/s41592-022-01467-6 PubMed 35606446
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