Skip to main content
Addgene

pBAD/His-miRFP670nano3
(Plasmid #184663)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184663 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBAD/His-B
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    miRFP670nano3
  • Species
    Synthetic
  • Insert Size (bp)
    441
  • Promoter araC
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBAD-fwd
  • 3′ sequencing primer pBAD-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Modifications to backbone: Several amino acids between His-tag and miRFP670nano were removed

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD/His-miRFP670nano3 was a gift from Vladislav Verkhusha (Addgene plasmid # 184663 ; http://n2t.net/addgene:184663 ; RRID:Addgene_184663)

  • For your References section:

    Single-domain near-infrared protein provides a scaffold for antigen-dependent fluorescent nanobodies. Oliinyk OS, Baloban M, Clark CL, Carey E, Pletnev S, Nimmerjahn A, Verkhusha VV. Nat Methods. 2022 May 23. pii: 10.1038/s41592-022-01467-6. doi: 10.1038/s41592-022-01467-6. 10.1038/s41592-022-01467-6 PubMed 35606446
Related items:
Commonly requested with: