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Addgene

pTriEx4_Rap1
(Plasmid #184662)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184662 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTriEx4
  • Backbone size w/o insert (bp) 5159
  • Total vector size (bp) 6359
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    rap1
  • Species
    M. musculus (mouse)
  • Promoter CMV

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CTGTAAATTACATTTTATTTACAATCAAAGGAG
  • 3′ sequencing primer CCTGAGGTTAATCACTTAGTGATG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTriEx4_Rap1 was a gift from Agnel Sfeir (Addgene plasmid # 184662 ; http://n2t.net/addgene:184662 ; RRID:Addgene_184662)

  • For your References section:

    Rap1 regulates TIP60 function during fate transition between two-cell-like and pluripotent states. Barry RM, Sacco O, Mameri A, Stojaspal M, Kartsonis W, Shah P, De Ioannes P, Hofr C, Cote J, Sfeir A. Genes Dev. 2022 Feb 24. pii: gad.349039.121. doi: 10.1101/gad.349039.121. 10.1101/gad.349039.121 PubMed 35210222
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