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pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA
(Plasmid #184636)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184636 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA
  • Backbone manufacturer
    Addgene #20297
  • Modifications to backbone
    3 copies of the influenza hemagglutinin (HA) coding sequence are inserted at the C-terminus
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gat1
  • Species
    M. musculus (mouse)
  • Mutation
    Gat1 gene with Arginine 69 changed to Lysine, to impair transport function
  • GenBank ID
    NM_178703.4
  • Entrez Gene
    Slc6a1 (a.k.a. A730043E01, GABATHG, GABATR, GAT-1, Gabt, Gabt1, Gat1, XT-1, Xtrp1)
  • Promoter EF1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA was a gift from Nicolas Tritsch (Addgene plasmid # 184636 ; http://n2t.net/addgene:184636 ; RRID:Addgene_184636)

  • For your References section:

    Inhibitory co-transmission from midbrain dopamine neurons relies on presynaptic GABA uptake. Melani R, Tritsch NX. Cell Rep. 2022 Apr 19;39(3):110716. doi: 10.1016/j.celrep.2022.110716. 10.1016/j.celrep.2022.110716 PubMed 35443174
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