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Addgene

pcDNA 3.1 KBTBD4 R313PRR
(Plasmid #184629)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184629 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N-terminal 3xFlag tagged KBTBD4 R313PRR
  • Alt name
    Kelch Repeat And BTB Domain Containing 4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1557
  • Mutation
    R313PRR
  • Entrez Gene
    KBTBD4 (a.k.a. BKLHD4, HSPC252)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA 3.1 KBTBD4 R313PRR was a gift from Vincenzo D'Angiolella (Addgene plasmid # 184629 ; http://n2t.net/addgene:184629 ; RRID:Addgene_184629)

  • For your References section:

    Disease-associated KBTBD4 mutations in medulloblastoma elicit neomorphic ubiquitylation activity to promote CoREST degradation. Chen Z, Ioris RM, Richardson S, Van Ess AN, Vendrell I, Kessler BM, Buffa FM, Busino L, Clifford SC, Bullock AN, D'Angiolella V. Cell Death Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi: 10.1038/s41418-022-00983-4. 10.1038/s41418-022-00983-4 PubMed 35379950
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