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pCW57.1 KBTBD4 P311PP
(Plasmid #184625)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184625 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCW57.1
  • Vector type
    Mammalian Expression, Lentiviral ; Doxycycline inducible
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    N-terminal Flag tagged KBTBD4 P311PP
  • Alt name
    Kelch Repeat And BTB Domain Containing 4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1557
  • Mutation
    P311PP
  • Entrez Gene
    KBTBD4 (a.k.a. BKLHD4, HSPC252)
  • Promoter TRE promoter, Tet ON

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer LNCX (5'-AGCTCGTTTAGTGAACCGTCAGATC-3')
  • 3′ sequencing primer hPGK Rev - (5'-gaaaaggcgcaaccccaacc-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCW57.1 KBTBD4 P311PP was a gift from Vincenzo D'Angiolella (Addgene plasmid # 184625 ; http://n2t.net/addgene:184625 ; RRID:Addgene_184625)

  • For your References section:

    Disease-associated KBTBD4 mutations in medulloblastoma elicit neomorphic ubiquitylation activity to promote CoREST degradation. Chen Z, Ioris RM, Richardson S, Van Ess AN, Vendrell I, Kessler BM, Buffa FM, Busino L, Clifford SC, Bullock AN, D'Angiolella V. Cell Death Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi: 10.1038/s41418-022-00983-4. 10.1038/s41418-022-00983-4 PubMed 35379950
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