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Addgene

BMP10 N67Q N131Q D4G (S2-2)
(Plasmid #184622)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184622 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pExpreS2-2
  • Backbone size w/o insert (bp) 2799
  • Vector type
    Insect Expression
  • Selectable markers
    G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BMP10
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1475
  • Mutation
    N67Q, N131Q, ARIRR(312-316) replaced with G
  • Entrez Gene
    BMP10
  • Tag / Fusion Protein
    • 8x His - streptavidin binding peptide (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba1 (unknown if destroyed)
  • 3′ cloning site Not1 (unknown if destroyed)
  • 5′ sequencing primer CGT CTA CGG AGC GAC AAT TC
  • 3′ sequencing primer CAC GCG CTT GAA AGG AGT GT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BMP10 N67Q N131Q D4G (S2-2) was a gift from Timothy Springer (Addgene plasmid # 184622 ; http://n2t.net/addgene:184622 ; RRID:Addgene_184622)

  • For your References section:

    Protection of the Prodomain alpha1-Helix Correlates with Latency in the Transforming Growth Factor-beta Family. Le VQ, Iacob RE, Zhao B, Su Y, Tian Y, Toohey C, Engen JR, Springer TA. J Mol Biol. 2022 Mar 15;434(5):167439. doi: 10.1016/j.jmb.2021.167439. Epub 2022 Jan 4. 10.1016/j.jmb.2021.167439 PubMed 34990654
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