pCIS.MTRIA-OX
(Plasmid
#184615)
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PurposeFor expression of a fluorescent sensor for orexin in mammalian cells
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184615 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCIS
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMTRIA-OX
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Alt nameOX biosensor
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SpeciesSynthetic
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Insert Size (bp)1896
- Promoter CAG promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2021.07.30.454450 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCIS.MTRIA-OX was a gift from Hiroshi Hibino & Daisuke Ino (Addgene plasmid # 184615 ; http://n2t.net/addgene:184615 ; RRID:Addgene_184615) -
For your References section:
A fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain. Ino D, Tanaka Y, Hibino H, Nishiyama M. Nat Methods. 2022 Sep 22. pii: 10.1038/s41592-022-01597-x. doi: 10.1038/s41592-022-01597-x. 10.1038/s41592-022-01597-x PubMed 36138174