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Addgene

pCIS.MTRIA-ENK
(Plasmid #184604)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184604 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCIS
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MTRIA-ENK
  • Alt name
    ENK biosensor
  • Species
    Synthetic
  • Insert Size (bp)
    1845
  • Promoter CAG promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.07.30.454450 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIS.MTRIA-ENK was a gift from Hiroshi Hibino & Daisuke Ino (Addgene plasmid # 184604 ; http://n2t.net/addgene:184604 ; RRID:Addgene_184604)

  • For your References section:

    A fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain. Ino D, Tanaka Y, Hibino H, Nishiyama M. Nat Methods. 2022 Sep 22. pii: 10.1038/s41592-022-01597-x. doi: 10.1038/s41592-022-01597-x. 10.1038/s41592-022-01597-x PubMed 36138174
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