Skip to main content
Addgene

pAAV.EF1a.FLEX.MTRIA-OT
(Plasmid #184595)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184595 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAAV.EF1a.FLEX
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MTRIA-OT
  • Alt name
    OT biosensor
  • Species
    Synthetic
  • Insert Size (bp)
    1833
  • Promoter Human elongation factor-1 alpha promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2021.07.30.454450 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV.EF1a.FLEX.MTRIA-OT was a gift from Hiroshi Hibino & Daisuke Ino (Addgene plasmid # 184595 ; http://n2t.net/addgene:184595 ; RRID:Addgene_184595)

  • For your References section:

    A fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain. Ino D, Tanaka Y, Hibino H, Nishiyama M. Nat Methods. 2022 Sep 22. pii: 10.1038/s41592-022-01597-x. doi: 10.1038/s41592-022-01597-x. 10.1038/s41592-022-01597-x PubMed 36138174
Related items:
Commonly requested with: