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K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized
(Plasmid #184563)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184563 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLJM60 (based on pLKO.1 and Lentihair)
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    C16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11
  • Alt name
    NPR3 like, GATOR1 complex subunit
  • Alt name
    Nitrogen permease regulator 3-like protein
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1710
  • Mutation
    All lysines were mutated to arginines. Codon-optimized for expression in human cells.
  • GenBank ID
    NM_001077350
  • Entrez Gene
    NPRL3 (a.k.a. C16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer (1) CMV Forward, (2) SP6
  • 3′ sequencing primer TAGTTTGTATGTCTGTTGCTATTA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized was a gift from Kacper Rogala (Addgene plasmid # 184563 ; http://n2t.net/addgene:184563 ; RRID:Addgene_184563)

  • For your References section:

    Structure of the nutrient-sensing hub GATOR2. Valenstein ML, Rogala KB, Lalgudi PV, Brignole EJ, Gu X, Saxton RA, Chantranupong L, Kolibius J, Quast JP, Sabatini DM. Nature. 2022 Jul;607(7919):610-616. doi: 10.1038/s41586-022-04939-z. Epub 2022 Jul 13. 10.1038/s41586-022-04939-z PubMed 35831510
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