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Flag-MIOS(1-875) in pRK5
(Plasmid #184561)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184561 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Backbone manufacturer
    Genentech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MIOS
  • Alt name
    Meiosis regulator for oocyte development
  • Alt name
    Missing oocyte meiosis regulator homolog
  • Alt name
    GATOR complex protein MIOS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2628
  • GenBank ID
    NM_001370076
  • Entrez Gene
    MIOS (a.k.a. MIO, Sea4, Yulink)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer (1) CMV Forward, (2) SP6
  • 3′ sequencing primer EBV Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-MIOS(1-875) in pRK5 was a gift from Kacper Rogala (Addgene plasmid # 184561 ; http://n2t.net/addgene:184561 ; RRID:Addgene_184561)

  • For your References section:

    Structure of the nutrient-sensing hub GATOR2. Valenstein ML, Rogala KB, Lalgudi PV, Brignole EJ, Gu X, Saxton RA, Chantranupong L, Kolibius J, Quast JP, Sabatini DM. Nature. 2022 Jul;607(7919):610-616. doi: 10.1038/s41586-022-04939-z. Epub 2022 Jul 13. 10.1038/s41586-022-04939-z PubMed 35831510
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