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PurposeAAV packaging plasmid, expressing Rep2 and AAV-MG1.2 capsid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184541 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV helper vector
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Vector typeAAV ; helper vector for packaging
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAAV-MG1.2 capsid
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SpeciesSynthetic
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Insert Size (bp)2232
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Mutationchanges on AAV9 capsid: K449R, Q588T; insertion of EPPKTTS between T588 and A589
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
rAAV2/MG1.2 was a gift from Minmin Luo (Addgene plasmid # 184541 ; http://n2t.net/addgene:184541 ; RRID:Addgene_184541) -
For your References section:
Directed evolution of adeno-associated virus for efficient gene delivery to microglia. Lin R, Zhou Y, Yan T, Wang R, Li H, Wu Z, Zhang X, Zhou X, Zhao F, Zhang L, Li Y, Luo M. Nat Methods. 2022 Jul 25. pii: 10.1038/s41592-022-01547-7. doi: 10.1038/s41592-022-01547-7. 10.1038/s41592-022-01547-7 PubMed 35879607