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Addgene

pHACK-QF2-AgU6-mCherry
(Plasmid #184530)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184530 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    T2A-QF2
  • Total vector size (bp) 8180
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    QF2
  • Species
    D. melanogaster (fly), Synthetic
  • Insert Size (bp)
    1053
  • Promoter Anopheles U6 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bbsl (unknown if destroyed)
  • 3′ cloning site Xho1 (unknown if destroyed)
  • 5′ sequencing primer GTTTGAATTGAATTGTCGCTCCGTAGACGAAGC
  • 3′ sequencing primer GCTTCGTCTACGGAGCGACAATTCAATTCAAAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHACK-QF2-AgU6-mCherry was a gift from Christopher Potter (Addgene plasmid # 184530 ; http://n2t.net/addgene:184530 ; RRID:Addgene_184530)

  • For your References section:

    A spatial map of antennal-expressed ionotropic receptors in the malaria mosquito. Raji JI, Konopka JK, Potter CJ. Cell Rep. 2023 Feb 10;42(2):112101. doi: 10.1016/j.celrep.2023.112101. 10.1016/j.celrep.2023.112101 PubMed 36773296
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