p8291 MSCV-Neo C-HA 18E7 ΔDLLC
(Plasmid
#184523)
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PurposeExpression of HPV18 E7 ΔDLLC
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184523 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneMSCV
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Vector typeRetroviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHPV18 E7 ΔDLLC
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SpeciesHuman papillomavirus
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Mutationdeletion of DLLC
- Promoter MSCV LTR
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p8291 MSCV-Neo C-HA 18E7 ΔDLLC was a gift from Elizabeth White (Addgene plasmid # 184523 ; http://n2t.net/addgene:184523 ; RRID:Addgene_184523) -
For your References section:
YAP1 activation by human papillomavirus E7 promotes basal cell identity in squamous epithelia. Hatterschide J, Castagnino P, Kim HW, Sperry SM, Montone KT, Basu D, White EA. Elife. 2022 Feb 16;11. pii: 75466. doi: 10.7554/eLife.75466. 10.7554/eLife.75466 PubMed 35170430