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Addgene

p8130 MSCV GFP Puro C-FlagHA
(Plasmid #184521)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184521 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP
  • Species
    Synthetic
  • Promoter MSCV LTR
  • Tag / Fusion Protein
    • Flag, HA (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p8130 MSCV GFP Puro C-FlagHA was a gift from Elizabeth White (Addgene plasmid # 184521 ; http://n2t.net/addgene:184521 ; RRID:Addgene_184521)

  • For your References section:

    YAP1 activation by human papillomavirus E7 promotes basal cell identity in squamous epithelia. Hatterschide J, Castagnino P, Kim HW, Sperry SM, Montone KT, Basu D, White EA. Elife. 2022 Feb 16;11. pii: 75466. doi: 10.7554/eLife.75466. 10.7554/eLife.75466 PubMed 35170430
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