pCI pHluorin Syt11
(Plasmid #184512)

• Purpose: Mammalian expression of Synaptotagmin 11 with pH-sensitive fluorescent protein
• Depositing Lab: Edwin Chapman
• Publication: Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. doi: 10.1091/mbc.E11-08-0707. Epub 2012 Mar 7.

Backbone

• Vector backbone: pCI
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4006
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Synaptotagmin 11
• Species: M. musculus (mouse)
• Insert Size (bp): 2151
• Entrez Gene: Syt11 (a.k.a. AI851970, 1500004A13Rik, 3632445O20Rik, 5430404N14Rik, 6530420C11Rik)
• Tag / Fusion Protein:
  • Super Ecliptic pHluorin (N terminal on backbone)

Cloning Information

• Cloning method: Unknown

Resource Information

• Supplemental Documents:
  • pCI pHluorin Syt11.gb
• A portion of this plasmid was derived from a plasmid made by: Mitsunori Fukuda, Tohoku University

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The first codon of the Synpatotagmin has been removed