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Addgene

pCI pHluorin Syt8
(Plasmid #184509)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184509 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4006
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Synaptotagmin 8
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2028
  • Entrez Gene
    Syt8 (a.k.a. MGC124104, MGC124105)
  • Tag / Fusion Protein
    • Super Ecliptic pHluorin (N terminal on backbone)

Cloning Information

  • Cloning method Unknown

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Mitsunori Fukuda, Tohoku University

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The first codon of the Synpatotagmin has been removed

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI pHluorin Syt8 was a gift from Edwin Chapman (Addgene plasmid # 184509 ; http://n2t.net/addgene:184509 ; RRID:Addgene_184509)

  • For your References section:

    Axonal and dendritic synaptotagmin isoforms revealed by a pHluorin-syt functional screen. Dean C, Dunning FM, Liu H, Bomba-Warczak E, Martens H, Bharat V, Ahmed S, Chapman ER. Mol Biol Cell. 2012 May;23(9):1715-27. doi: 10.1091/mbc.E11-08-0707. Epub 2012 Mar 7. 10.1091/mbc.E11-08-0707 PubMed 22398727
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