pCI pHluorin Syt4
(Plasmid
#184505)
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PurposeMammalian expression of Synaptotagmin 4 with pH-sensitive fluorescent protein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 184505 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCI
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4006
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSynaptotagmin 4
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SpeciesR. norvegicus (rat)
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Insert Size (bp)2136
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Entrez GeneSyt4
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Tag
/ Fusion Protein
- Super Ecliptic pHluorin (N terminal on backbone)
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byB. Hillbush, Roche Research Center; Component Syt4 was provided by Dr. Herschman
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The first codon of the Synpatotagmin has been removed
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI pHluorin Syt4 was a gift from Edwin Chapman (Addgene plasmid # 184505 ; http://n2t.net/addgene:184505 ; RRID:Addgene_184505) -
For your References section:
Axonal and dendritic synaptotagmin isoforms revealed by a pHluorin-syt functional screen. Dean C, Dunning FM, Liu H, Bomba-Warczak E, Martens H, Bharat V, Ahmed S, Chapman ER. Mol Biol Cell. 2012 May;23(9):1715-27. doi: 10.1091/mbc.E11-08-0707. Epub 2012 Mar 7. 10.1091/mbc.E11-08-0707 PubMed 22398727
