GST-30A
(Plasmid
#1845)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1845 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-2TK
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 4969
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFBP30 WW-A
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Alt nameWW domain
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Alt nameformin-binding protein 30
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SpeciesM. musculus (mouse)
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GenBank IDU40750
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Entrez GeneFnbp4 (a.k.a. FBP30, Fnbp30, mKIAA1014)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
(Leder #C-1094)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST-30A was a gift from Philip Leder (Addgene plasmid # 1845 ; http://n2t.net/addgene:1845 ; RRID:Addgene_1845) -
For your References section:
A novel pro-Arg motif recognized by WW domains. Bedford MT, Sarbassova D, Xu J, Leder P, Yaffe MB. J Biol Chem 2000 Apr 7;275(14):10359-69. 10.1074/jbc.275.14.10359 PubMed 10744724