pYFAC-ubi-Y-pyrG
(Plasmid #184497)

• Purpose: (Empty Backbone) AMA1 based vector for optimised episomal expression in filamentous fungi, with increased selection stringency using the modified ubi-Y-pyrG marker (high stringency).
• Depositing Lab: Yit Heng Chooi
• Publication: Roux et al Microb Biotechnol. 2024 Sep;17(9):e70010. doi: 10.1111/1751-7915.70010.

Backbone

• Vector backbone: pYFAC-ubi-Y-pyrG
• Backbone size (bp): 14542
• Modifications to backbone: Contains autotrophy marker pyrG fused to a ubi-Y degron for more stringent plasmid selection
• Vector type: Synthetic Biology ; Expression in Aspergillus and related filamentous fungi
• Selectable markers: URA3 ; ubi-Y-pyrG

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For improved transformation efficiency, use a DH10B E coli, and electroporation.
• Copy number: High Copy

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • i152-pkw-2u-ub-y.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Derived from pYFAC (Addgene Plasmid #168982), incorporating ubi-Y in the N-terminal of pyrG for more stringent selection. Filamentous fungi AMA1 vector , which also supports growth in E coli and yeast.