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iMbody_pSANG10-3F
(Plasmid #184496)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184496 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSANG10-3F
  • Backbone manufacturer
    John McCafferty (Addgene plasmid # 39264)
  • Backbone size w/o insert (bp) 5438
  • Total vector size (bp) 5780
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Must be transformed into BL21(DE3) bacteria and grown at 30 degrees for iMbody production.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    iMbody
  • Species
    Synthetic
  • Insert Size (bp)
    357
  • Tags / Fusion Proteins
    • 6xHis (C terminal on backbone)
    • 3xFLAG (C terminal on backbone)
    • pelB leader (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminal
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    iMbody_pSANG10-3F was a gift from Marcel Dinger (Addgene plasmid # 184496 ; http://n2t.net/addgene:184496 ; RRID:Addgene_184496)

  • For your References section:

    Protocol for the production and purification of an i-Motif-specific nanobody. Zeraati M, Ross SE, Aghaei B, Rajal AG, King C, Dinger ME. STAR Protoc. 2023 Nov 21;4(4):102729. doi: 10.1016/j.xpro.2023.102729. 10.1016/j.xpro.2023.102729 PubMed 37995194
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