pDONR221-PARP7
(Plasmid
#184474)
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PurposeFor gateway cloning of PARP7
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184474 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
- Total vector size (bp) 4521
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Vector typeGateway cloning
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePARP7
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Alt nametiparp
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SpeciesH. sapiens (human)
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Insert Size (bp)1974
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Entrez GeneTIPARP (a.k.a. ARTD14, PARP7, pART14)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2022.02.22.481411 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221-PARP7 was a gift from Karla Feijs (Addgene plasmid # 184474 ; http://n2t.net/addgene:184474 ; RRID:Addgene_184474) -
For your References section:
Protein and RNA ADP-ribosylation detection is influenced by sample preparation and reagents used. Weixler L, Ikenga NJ, Voorneveld J, Aydin G, Bolte TM, Momoh J, Butepage M, Golzmann A, Luscher B, Filippov DV, Zaja R, Feijs KL. Life Sci Alliance. 2022 Nov 11;6(1):e202201455. doi: 10.26508/lsa.202201455. 10.26508/lsa.202201455 PubMed 36368907