PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9
(Plasmid #184464)

• Purpose: Plasmid encoding PRDM9-Cas9 fusion under CMV promoter
• Depositing Lab: Jennifer Doudna
• Publication: Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119(49):e2204259119. doi: 10.1073/pnas.2204259119. Epub 2022 Dec 2.

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 4790
• Total vector size (bp): 10907
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9
• Species: H. sapiens (human)
• Insert Size (bp): 6120
• Entrez Gene: PRDM9 (a.k.a. KMT8B, MEISETZ, MSBP3, PFM6, ZNF899)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: CTCTAGAGCCTCTGCTAACC
• 3′ sequencing primer: GCCAGAAGTCAGATGCTCAAGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry