T4 Lysozyme R96A WT*
(Plasmid
#18445)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18445 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHS1403
- Backbone size w/o insert (bp) 5000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameT4 Lysozyme
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SpeciesBacteriophage T4
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Insert Size (bp)600
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MutationR96A WT*
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GenBank IDNP_049736
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Entrez Genee (a.k.a. T4p123)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer na
- 3′ sequencing primer GGCGTATCACGAGGCCC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
T4 Lysozyme R96A WT* was a gift from Brian Matthews (Addgene plasmid # 18445 ; http://n2t.net/addgene:18445 ; RRID:Addgene_18445)