pVT36b
(Plasmid
#184437)
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Purpose(Empty Backbone) CRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1’-tRNA_Leu promoter, and sgRNA scaffold
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 184437 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepVT36b
- Backbone size (bp) 9550
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Vector typeYeast Expression, CRISPR
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVT36b was a gift from Huimin Zhao (Addgene plasmid # 184437 ; http://n2t.net/addgene:184437 ; RRID:Addgene_184437) -
For your References section:
Development of a CRISPR/Cas9-Based Tool for Gene Deletion in Issatchenkia orientalis. Tran VG, Cao M, Fatma Z, Song X, Zhao H. mSphere. 2019 Jun 26;4(3). pii: 4/3/e00345-19. doi: 10.1128/mSphere.00345-19. 10.1128/mSphere.00345-19 PubMed 31243078
