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Addgene

pCS2+/N-Halo
(Plasmid #184417)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184417 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size (bp) 4238
  • Vector type
    for mRNA synthesis
  • Tag / Fusion Protein
    • Halo-tag (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Mutation
    Codon optimized for expression in Xenopus laevis at position 82, 121, 154, 166, 403, 511, 517, 535, 571, 643, 655, 700, 742, 802, 832, 856, 868, 871
  • Tag / Fusion Protein
    • Halo-tag (N terminal on backbone)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Promega: Halo-tag codon optimized for expression in Xenopus laevis

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+/N-Halo was a gift from Ann Miller (Addgene plasmid # 184417 ; http://n2t.net/addgene:184417 ; RRID:Addgene_184417)

  • For your References section:

    SNAP- and Halo-tagging and dye introduction protocol for live microscopy in Xenopus embryos. Dudley CE, van den Goor L, Miller AL. STAR Protoc. 2022 Aug 18;3(3):101622. doi: 10.1016/j.xpro.2022.101622. eCollection 2022 Sep 16. 10.1016/j.xpro.2022.101622 PubMed 36035797
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