pCS2+/N-SNAPf
(Plasmid
#184415)
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Purpose(Empty Backbone) vector backbone to clone insert downstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 184415 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
- Backbone size (bp) 4235
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Vector typefor mRNA synthesis
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Tag
/ Fusion Protein
- SNAPf-tag (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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MutationCodon optimized for expression in Xenopus laevis at position 436
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Tag
/ Fusion Protein
- SNAPf-tag (N terminal on backbone)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byNEB: SNAPf-tag codon optimized for expression in Xenopus laevis
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2+/N-SNAPf was a gift from Ann Miller (Addgene plasmid # 184415 ; http://n2t.net/addgene:184415 ; RRID:Addgene_184415) -
For your References section:
SNAP- and Halo-tagging and dye introduction protocol for live microscopy in Xenopus embryos. Dudley CE, van den Goor L, Miller AL. STAR Protoc. 2022 Aug 18;3(3):101622. doi: 10.1016/j.xpro.2022.101622. eCollection 2022 Sep 16. 10.1016/j.xpro.2022.101622 PubMed 36035797
Map uploaded by the depositor.
