pAAV-TRE-GCaMP6s-WPRE-sv40
(Plasmid #184414)

• Purpose: AAV expression of Fluorescent calcium reporter, GCaMP6s from TRE promoter
• Depositing Lab: Marylka Yoe Uusisaari
• Publication: Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:825056. doi: 10.3389/fncel.2022.825056. eCollection 2022.

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 3101
• Total vector size (bp): 5819
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GCaMP6s
• Species: Synthetic
• Insert Size (bp): 2718
• Promoter: TRE

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: GTATGGATCCacccgccaccATGggttctcatc
• 3′ sequencing primer: GATAAGCTTGATATCGAATTCTCActtcgctg

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry