pAAV-TRE-GCaMP6s-WPRE-sv40
(Plasmid
#184414)
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PurposeAAV expression of Fluorescent calcium reporter, GCaMP6s from TRE promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184414 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 3101
- Total vector size (bp) 5819
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGCaMP6s
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SpeciesSynthetic
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Insert Size (bp)2718
- Promoter TRE
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer GTATGGATCCacccgccaccATGggttctcatc
- 3′ sequencing primer GATAAGCTTGATATCGAATTCTCActtcgctg (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-TRE-GCaMP6s-WPRE-sv40 was a gift from Marylka Yoe Uusisaari (Addgene plasmid # 184414 ; http://n2t.net/addgene:184414 ; RRID:Addgene_184414) -
For your References section:
Designing AAV Vectors for Monitoring the Subtle Calcium Fluctuations of Inferior Olive Network in vivo. Dorgans K, Guo D, Kurima K, Wickens J, Uusisaari MY. Front Cell Neurosci. 2022 Apr 27;16:825056. doi: 10.3389/fncel.2022.825056. eCollection 2022. 10.3389/fncel.2022.825056 PubMed 35573836