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pAAV-TRE-GCaMP6s-WPRE-sv40
(Plasmid #184414)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184414 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 3101
  • Total vector size (bp) 5819
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GCaMP6s
  • Species
    Synthetic
  • Insert Size (bp)
    2718
  • Promoter TRE

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GTATGGATCCacccgccaccATGggttctcatc
  • 3′ sequencing primer GATAAGCTTGATATCGAATTCTCActtcgctg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-TRE-GCaMP6s-WPRE-sv40 was a gift from Marylka Yoe Uusisaari (Addgene plasmid # 184414 ; http://n2t.net/addgene:184414 ; RRID:Addgene_184414)

  • For your References section:

    Designing AAV Vectors for Monitoring the Subtle Calcium Fluctuations of Inferior Olive Network in vivo. Dorgans K, Guo D, Kurima K, Wickens J, Uusisaari MY. Front Cell Neurosci. 2022 Apr 27;16:825056. doi: 10.3389/fncel.2022.825056. eCollection 2022. 10.3389/fncel.2022.825056 PubMed 35573836
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