pAAV-Susd4(3.7)-EGFP-sv40
(Plasmid #184413)

• Purpose: AAV expression of tTA from mouse sushi domain containing 4 (Susd4) gene promoter.
• Depositing Lab: Marylka Yoe Uusisaari
• Publication: Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:825056. doi: 10.3389/fncel.2022.825056. eCollection 2022.

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 3074
• Total vector size (bp): 7822
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mouse Susd4 gene promoter-EGFP
• Species: M. musculus (mouse)
• GenBank ID: MGI:2138351
• Entrez Gene: Susd4 (a.k.a. AI848994, E430021N18Rik, N28096)
• Promoter: Mouse Susd4 gene
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: EcoRI (destroyed during cloning)
• 5′ sequencing primer: atgaAGATCTccattatttataaactatgtgg
• 3′ sequencing primer: atgaCAATTGgcaagcgagcgagcgagcaagacagaggagc

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry