TTR C10S S85C
(Plasmid
#184410)
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Purposebacterial expression of TTR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184410 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepet29
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Backbone manufactureremdmillipore
- Backbone size w/o insert (bp) 5371
- Total vector size (bp) 5758
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTTR
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SpeciesH. sapiens (human)
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Insert Size (bp)387
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MutationC10S, S85C
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Entrez GeneTTR (a.k.a. ATTR, CTS, CTS1, HEL111, HsT2651, PALB, TBPA, TTN)
- Promoter T7
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byWe made mutations on the wild type construct received from the Kelly lab. Description of wild type TTR plasmid generation in supplementary info of this reference : https://pubs.acs.org/doi/abs/10.1021/cb5004477
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TTR C10S S85C was a gift from Peter Wright (Addgene plasmid # 184410 ; http://n2t.net/addgene:184410 ; RRID:Addgene_184410) -
For your References section:
Kinetic analysis of the multistep aggregation pathway of human transthyretin. Sun X, Dyson HJ, Wright PE. Proc Natl Acad Sci U S A. 2018 Jul 3;115(27):E6201-E6208. doi: 10.1073/pnas.1807024115. Epub 2018 Jun 18. 10.1073/pnas.1807024115 PubMed 29915031