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3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y
(Plasmid #184406)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184406 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETcon
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 Beta Spike receptor binding domain (RBD)
  • Species
    SARS-CoV-2 virus
  • Mutation
    K417N+E484K+N501Y
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • c-Myc (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

bioRxiv 10.1101/2022.02.24.481899v1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y was a gift from Jesse Bloom (Addgene plasmid # 184406 ; http://n2t.net/addgene:184406 ; RRID:Addgene_184406)

  • For your References section:

    Shifting mutational constraints in the SARS-CoV-2 receptor-binding domain during viral evolution. Starr TN, Greaney AJ, Hannon WW, Loes AN, Hauser K, Dillen JR, Ferri E, Farrell AG, Dadonaite B, McCallum M, Matreyek KA, Corti D, Veesler D, Snell G, Bloom JD. Science. 2022 Jun 28:eabo7896. doi: 10.1126/science.abo7896. 10.1126/science.abo7896 PubMed 35762884
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