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pAT15541_dCBE-SuperFi-Cas9
(Plasmid #184371)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184371 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCBE-SuperFi-Cas9
  • Alt name
    nuclease inactive SuperFi-Cas9 fused with APOBEC cytosine deaminase
  • Species
    S. pyogenes
  • Mutation
    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, K1031D
  • Promoter EF-1α core promoter
  • Tags / Fusion Proteins
    • SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)
    • 3X FLAG (N terminal on insert)
    • UGI (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAT15541_dCBE-SuperFi-Cas9 was a gift from Ervin Welker (Addgene plasmid # 184371 ; http://n2t.net/addgene:184371 ; RRID:Addgene_184371)

  • For your References section:

    SuperFi-Cas9 exhibits remarkable fidelity but severely reduced activity yet works effectively with ABE8e. Kulcsar PI, Talas A, Ligeti Z, Krausz SL, Welker E. Nat Commun. 2022 Nov 11;13(1):6858. doi: 10.1038/s41467-022-34527-8. 10.1038/s41467-022-34527-8 PubMed 36369279
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