Skip to main content
Addgene

pPIK16045_pX330_Flag-SuperFi-Cas9
(Plasmid #184370)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184370 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    px330
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SuperFi-Cas9
  • Species
    S. pyogenes
  • Mutation
    Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, K1031D
  • Promoter CBh
  • Tags / Fusion Proteins
    • 3X FLAG (N terminal on insert)
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPIK16045_pX330_Flag-SuperFi-Cas9 was a gift from Ervin Welker (Addgene plasmid # 184370 ; http://n2t.net/addgene:184370 ; RRID:Addgene_184370)

  • For your References section:

    SuperFi-Cas9 exhibits remarkable fidelity but severely reduced activity yet works effectively with ABE8e. Kulcsar PI, Talas A, Ligeti Z, Krausz SL, Welker E. Nat Commun. 2022 Nov 11;13(1):6858. doi: 10.1038/s41467-022-34527-8. 10.1038/s41467-022-34527-8 PubMed 36369279
Related items:
Commonly requested with: