pCMV10-3xFLAG-prs-XK
(Plasmid
#184369)
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PurposeExpress human XK in Expi293 cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184369 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV10
- Backbone size w/o insert (bp) 6281
- Total vector size (bp) 7616
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Modifications to backbonedigested with NotI and BamHI
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHuman XK
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Alt nameXK-related protein1
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SpeciesH. sapiens (human)
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Insert Size (bp)1335
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GenBank IDNC_000023.11
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Entrez GeneXK (a.k.a. KX, NA, NAC, X1k, XKR1)
- Promoter CMV
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Tag
/ Fusion Protein
- 3xFLAG-PreScission cleavage site (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ccaggggccccttgcggccgcgATGAAATTCCCGGCCTCG
- 3′ sequencing primer agggatgccacccgggatccTTAAGCAGAGCAGAGATCTTC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV10-3xFLAG-prs-XK was a gift from Karin Reinisch (Addgene plasmid # 184369 ; http://n2t.net/addgene:184369 ; RRID:Addgene_184369) -
For your References section:
Structural and biochemical insights into lipid transport by VPS13 proteins. Adlakha J, Hong Z, Li P, Reinisch KM. J Cell Biol. 2022 Jun 6;221(5):e202202030. doi: 10.1083/jcb.202202030. Epub 2022 Mar 31. 10.1083/jcb.202202030 PubMed 35357422