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Addgene

pPT24-mtnluc
(Plasmid #184339)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184339 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPT24
  • Backbone size w/o insert (bp) 5453
  • Total vector size (bp) 6173
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    nanoluciferase recoded for mitochondrial expression flanked by COX2 UTRs
  • Alt name
    mtnLuc
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    720

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer aaa
  • 3′ sequencing primer aaa
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPT24-mtnluc was a gift from Martin Ott (Addgene plasmid # 184339 ; http://n2t.net/addgene:184339 ; RRID:Addgene_184339)

  • For your References section:

    Incorporation of reporter genes into mitochondrial DNA in budding yeast. Rzepka M, Suhm T, Ott M. STAR Protoc. 2022 May 25;3(2):101359. doi: 10.1016/j.xpro.2022.101359. eCollection 2022 Jun 17. 10.1016/j.xpro.2022.101359 PubMed 35634362
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