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Addgene

pDisplay-V5-Nxph1
(Plasmid #184336)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184336 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDisplay-AP-CFP-TM
  • Backbone manufacturer
    Alice Ting Lab / Addgene_#20861
  • Modifications to backbone
    HA-tag was exchanged for V5-tag
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    neurexophilin-1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    759
  • Entrez Gene
    Nxph1 (a.k.a. C130005L03Rik)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5-tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDisplay-V5-Nxph1 was a gift from Peter Scheiffele (Addgene plasmid # 184336 ; http://n2t.net/addgene:184336 ; RRID:Addgene_184336)

  • For your References section:

    Targeted proteoform mapping uncovers specific Neurexin-3 variants required for dendritic inhibition. Hauser D, Behr K, Konno K, Schreiner D, Schmidt A, Watanabe M, Bischofberger J, Scheiffele P. Neuron. 2022 May 8. pii: S0896-6273(22)00362-2. doi: 10.1016/j.neuron.2022.04.017. 10.1016/j.neuron.2022.04.017 PubMed 35550065
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