pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(+)
(Plasmid #184334)

• Purpose: Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.
• Depositing Lab: Peter Scheiffele
• Publication: Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)00362-2. doi: 10.1016/j.neuron.2022.04.017.

Backbone

• Vector backbone: pDisplay-AP-CFP-TM
• Backbone manufacturer: Alice Ting Lab / Addgene_#20861
• Modifications to backbone: EGFP-2A was inserted after the CMV promoter in frame with the signal peptide
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Nrxn3-alpha
• Species: M. musculus (mouse)
• Insert Size (bp): 4353
• Entrez Gene: Nrxn3
• Promoter: CMV
• Tag / Fusion Protein:
  • HA-tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)3(+)4(+)6(+)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry