pcDNA3-NLS-YFP-CCTd
(Plasmid
#184325)
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PurposeMammalian expression of the nuclear localized YFP-CCTd(peptide of Cav1.3)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184325 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCCTd
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Alt namecleaved carboxyl-terminal fragment from CaV1.3 pore-forming subunit a1D
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SpeciesH. sapiens (human)
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Entrez GeneCACNA1D (a.k.a. CACH3, CACN4, CACNL1A2, CCHL1A2, Cav1.3, PASNA, SANDD)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-NLS-YFP-CCTd was a gift from Xiaodong Liu (Addgene plasmid # 184325 ; http://n2t.net/addgene:184325 ; RRID:Addgene_184325) -
For your References section:
Cytosolic peptides encoding Ca(V)1 C-termini downregulate the calcium channel activity-neuritogenesis coupling. Yang Y, Yu Z, Geng J, Liu M, Liu N, Li P, Hong W, Yue S, Jiang H, Ge H, Qian F, Xiong W, Wang P, Song S, Li X, Fan Y, Liu X. Commun Biol. 2022 May 19;5(1):484. doi: 10.1038/s42003-022-03438-1. 10.1038/s42003-022-03438-1 PubMed 35589958