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Addgene

I250L
(Plasmid #184306)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184306 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPICZ B
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3328
  • Vector type
    Yeast Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Act5C
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1431
  • Mutation
    I250L
  • Entrez Gene
    Act5C (a.k.a. Dmel_CG4027, A, A4V404_DROME, ACT, ACT1_DROME, ACT5C, Ac5C, Act, Act-5C, Act5, Act5c, Actin, Actin/BAP47, Actin5C, ArpA, BAP47, Bap47, CG4027, Dmel\CG4027, F-ACTIN, F-Actin, F-actin, G-actin, M32055, T11, act, act 5C, act5C, actin, actin5C, actomyosin, anon-EST:fe2D2, beta-Actin, beta-actin, beta-actin/Bap47, chrX:5748184..5748304, cyt5C, f-actin, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486)
  • Promoter AOX1
  • Tag / Fusion Protein
    • B-Thymosin followedby hexahistidine tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer 5'-GACTGGTTCCAATTGACAAGC-3'
  • 3′ sequencing primer 5'-GCAAATGGCATTCTGACATCC-3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    I250L was a gift from Michael Rosen (Addgene plasmid # 184306 ; http://n2t.net/addgene:184306 ; RRID:Addgene_184306)

  • For your References section:

    Bound nucleotide can control the dynamic architecture of monomeric actin. Ali R, Zahm JA, Rosen MK. Nat Struct Mol Biol. 2022 Apr;29(4):320-328. doi: 10.1038/s41594-022-00743-5. Epub 2022 Mar 24. 10.1038/s41594-022-00743-5 PubMed 35332323
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