pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Rxfp3
(Plasmid #184290)

• Purpose: A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Rxfp3
• Depositing Lab: Bernardo Sabatini
• Publication: Various Crispr/Cas9 plasmids (unpublished)

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Backbone

• Vector backbone: pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA
• Total vector size (bp): 7447
• Modifications to backbone: sgRNA targeting Rxfp3 was inserted via BsaI restriction digest
• Vector type: Mammalian Expression, AAV, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: sgRNA-Rxfp3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The backbone vector is from Addgene and was cloned and validated at the Genome Engineering & iPSC Center.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry