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PurposeAnti-mouse kappa chain specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)
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Depositing Labs
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 184286 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTXB1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namenbMmKappa-Tn5
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTXB1-nbMmKappa-Tn5 was a gift from New York Genome Center & Ivan Raimondi (Addgene plasmid # 184286 ; http://n2t.net/addgene:184286 ; RRID:Addgene_184286) -
For your References section:
Nanobody-tethered transposition enables multifactorial chromatin profiling at single-cell resolution. Stuart T, Hao S, Zhang B, Mekerishvili L, Landau DA, Maniatis S, Satija R, Raimondi I. Nat Biotechnol. 2022 Dec 19. doi: 10.1038/s41587-022-01588-5. 10.1038/s41587-022-01588-5 PubMed 36536150