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Addgene

pOPINOH11-A10
(Plasmid #184278)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184278 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOPINO
  • Backbone size w/o insert (bp) 5564
  • Total vector size (bp) 5592
  • Vector type
    Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use WK6 cells for expression
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Nanobody H11-A10
  • Species
    llama
  • Insert Size (bp)
    381
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOPINOH11-A10 was a gift from Ray Owens (Addgene plasmid # 184278 ; http://n2t.net/addgene:184278 ; RRID:Addgene_184278)

  • For your References section:

    Correlation between the binding affinity and the conformational entropy of nanobody SARS-CoV-2 spike protein complexes. Mikolajek H, Weckener M, Brotzakis ZF, Huo J, Dalietou EV, Le Bas A, Sormanni P, Harrison PJ, Ward PN, Truong S, Moynie L, Clare DK, Dumoux M, Dormon J, Norman C, Hussain N, Vogirala V, Owens RJ, Vendruscolo M, Naismith JH. Proc Natl Acad Sci U S A. 2022 Aug 2;119(31):e2205412119. doi: 10.1073/pnas.2205412119. Epub 2022 Jul 15. 10.1073/pnas.2205412119 PubMed 35858383
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