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Addgene

QUIN
(Plasmid #184267)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184267 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAIDE (a pCambia1300 drivative)
  • Backbone manufacturer
    Qu lab
  • Backbone size w/o insert (bp) 7869
  • Total vector size (bp) 14244
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CPSMV RNA1 cDNA with 4 introns
  • Species
    Cowpea severe mosaic virus
  • Mutation
    S1098P, K1651R
  • GenBank ID
    NC_003545.1
  • Promoter CaMV 35S with duplicated enhancer

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GAAACGACAATCTGATCCAAGCTCAA
  • 3′ sequencing primer GAGTTAGCTCACTCATTAGGCAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    QUIN was a gift from Feng Qu (Addgene plasmid # 184267 ; http://n2t.net/addgene:184267 ; RRID:Addgene_184267)

  • For your References section:

    A cowpea severe mosaic virus-based vector simplifies virus-induced gene silencing and foreign protein expression in soybean. Zaulda FA, Yang SH, Han J, Mlotshwa S, Dorrance A, Qu F. Plant Methods. 2022 Oct 28;18(1):116. doi: 10.1186/s13007-022-00950-7. 10.1186/s13007-022-00950-7 PubMed 36307846
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