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Addgene

ATX3-JD
(Plasmid #184247)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184247 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST17
  • Backbone manufacturer
    pDEST, Gateway T7 Vectors
  • Backbone size w/o insert (bp) 6354
  • Modifications to backbone
    Inclusion of a TEV cleavage site after the 6x HIS-tag
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ataxin-3 Josephin domain
  • Alt name
    ATXN3
  • Alt name
    SCA3
  • Alt name
    MJD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    615
  • Mutation
    Premature stop codon V183X
  • GenBank ID
    NM_004993.6
  • Entrez Gene
    ATXN3 (a.k.a. AT3, ATX3, JOS, MJD, MJD1, SCA3)
  • Promoter T7 promoter
  • Tags / Fusion Proteins
    • 6x His-Tag (N terminal on insert)
    • TEV cleavage site (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer T7- TAATACGACTCACTATAGGG
  • 3′ sequencing primer T7 term- GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ATX3-JD was a gift from Sandra Macedo Ribeiro (Addgene plasmid # 184247 ; http://n2t.net/addgene:184247 ; RRID:Addgene_184247)

  • For your References section:

    A Robust Assay to Monitor Ataxin-3 Amyloid Fibril Assembly. Figueiredo F, Lopes-Marques M, Almeida B, Matscheko N, Martins PM, Silva A, Macedo-Ribeiro S. Cells. 2022 Jun 19;11(12). pii: cells11121969. doi: 10.3390/cells11121969. 10.3390/cells11121969 PubMed 35741099
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